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For more information, please see full course syllabus of Microbiology
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Lecture Comments (2)

1 answer

Last reply by: Professor Catherine Carpenter Carpenter
Thu Apr 9, 2015 2:07 PM

Post by Cho Yu Lai on April 9, 2015

Hi there,
Got a question about the Malachite Green Spore test. Is the rationale behind the steps similar to the Gram stain and Acid fast test? But we don't use decolourizing solution such as ethanol to remove the Malachite green. Why mercurochrome can stain the vegetative cells into red? What is the rationale behind it?
Best wishes.

Bacterial Adaptation to Environment

  • Bacterial Adaptation to Living Inside and Outside Host
    • Environmental variations
      • Human host tissue
      • Levels of oxygenation
      • Levels of moisture
      • Exposure to antibiotic agents
  • Bacterial Endospores
    • Examples of bacteria which produce endospores
      • Clostridium botulinum
      • Bacillus anthracis
      • Clostridium tetani

Bacterial Adaptation to Environment

Lecture Slides are screen-captured images of important points in the lecture. Students can download and print out these lecture slide images to do practice problems as well as take notes while watching the lecture.

  • Intro 0:00
  • Bacterial Adaptation 0:13
    • Varied Tissues Within Human Host
    • Variable Levels of Oxygenation Both Inside and Outside of Host
    • Variable Levels of Moisture Both Inside and Outside Host
    • Survive Various Antibiotic and Other Types of Treatment
  • Variable Oxygen Environments 1:58
  • Bacterial Endospores 3:33
    • Clostridium Botulinum
    • Bacillus Anthracis
    • Clostridium Tetani
  • Botulism: Neurotoxin 5:10
  • Clostridium Botulinum 6:29
    • Gram Positive Rod-Shaped Bacteria That are Strictly Anaerobic
    • Produce Spores
    • Produces Paralysis
    • Toxin Can be Destroyed by Heating Food to 80 Degrees Celsius
  • Bacillus Anthracis 8:47
    • Produce Spores
    • Anthrax is Mostly a Disease of Herbivores
    • Weaponized Anthrax is Primarily Inhalation Form
  • Clostridium Tetani 11:50
    • Spores are Located in Solid and Can Colonize Gastrointestinal Tracts
    • Disease Uncommon
    • Toxin Produced During Growth Phase of Bacteria When Cell is Lysed
    • Toxin Blocks Release of GABA
    • Results in Paralysis
  • Example 1 15:38
  • Example 2 16:45
  • Example 3 18:01