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For more information, please see full course syllabus of Microbiology
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Laboratory Testing & Visualization

  • Laboratory Testing with Focus on Bacteria
    • Serology
    • Agglutination, ELISA, Western Blot
    • Flow cytometry
    • Genetics
  • Visualization
    • Microscopes and degree of magnification
    • Light microscopy
    • Electron microscopy
    • Atomic Power microscopy

Laboratory Testing & Visualization

Lecture Slides are screen-captured images of important points in the lecture. Students can download and print out these lecture slide images to do practice problems as well as take notes while watching the lecture.

  1. Intro
    • Laboratory Testing and Visualization
    • A Clinical Microbiology Lab Report Form
      • Generalized Tests for Microorganisms
      • A Clinical Microbiology Lab Report Form
        • Serology
        • Example of Serology Testing for HBV
          • Direct Agglutination Testing
          • ELISA Test
          • Western Blot
          • Flow Cytometry
          • Genetic Testing: DNA Fingerprinting
          • Pattern Matching to Determine Bacterial Strain
          • Instruments to Visualize Microorganisms
            • Light Microscope
            • Darkfield Microscopy
            • Planaria in Pond Water
              • Electron Microscope
              • Atomic Force Microscope
              • Atomic Force Imaging
                • Instruments to Visualize Microorganisms
                • Example 1
                  • Example 2
                    • Example 3
                      • Example 4
                        • Example 5
                          • Intro 0:00
                          • Laboratory Testing and Visualization 0:37
                            • Serology
                            • Visualization: Types of Microscopes
                          • A Clinical Microbiology Lab Report Form 1:57
                          • Generalized Tests for Microorganisms 2:36
                            • Morphological Characteristics
                            • Differential Staining
                            • Biochemical Tests
                          • A Clinical Microbiology Lab Report Form 4:19
                          • Serology 6:38
                            • Detect Levels of Antibodies
                            • Blood Serum
                            • Recent of Past Infection
                            • Differentiate Different Strains
                          • Example of Serology Testing for HBV 10:02
                          • Direct Agglutination Testing 12:52
                            • Visual Test
                            • Positive Results
                            • Antibodies Sufficient in Level
                          • ELISA Test 15:56
                            • Sandwich ELISA
                          • Western Blot 18:56
                            • Proteins are Positioned on the Filter so Antibodies Can Bind to the Antigens
                            • Filter is Then Washed with Patient's Serum
                            • Positive Test for Particular Microorganisms
                          • Flow Cytometry 21:09
                            • Used to Identify Bacteria Without Culturing the Bacteria
                            • Moving Fluid Containing Bacteria is Forced Through Small Opening
                            • Differences in Electrical Conductivity Between the Cells are Detected
                            • Results Distinguishing Three Different Species of Microorganisms
                          • Genetic Testing: DNA Fingerprinting 23:49
                            • Way to Specify and Differentiate Bacteria
                            • Some Produce Taxon
                            • Used as a Proxy for Microbial Cell Abundance
                            • Detailed Figure
                          • Pattern Matching to Determine Bacterial Strain 27:22
                            • Example
                            • Picture of That
                          • Instruments to Visualize Microorganisms 29:36
                          • Light Microscope 30:22
                            • Image
                          • Darkfield Microscopy 31:44
                            • An Illumination Technique Used to Enhance the Contrast in Unstained Samples
                            • How It Works
                          • Planaria in Pond Water 32:19
                          • Electron Microscope 32:55
                            • Uses Electron Beam to Illuminate a Specimen and Produce a Magnified Image
                            • Electron Microscopy
                            • Electron Microscope Image
                          • Atomic Force Microscope 34:41
                            • Manipulates Matter at the Nanoscale
                            • Atomic Force Microscopy Image
                          • Atomic Force Imaging 35:54
                          • Instruments to Visualize Microorganisms 37:02
                            • Light Microscopes
                          • Example 1 37:28
                          • Example 2 40:19
                          • Example 3 40:57
                          • Example 4 42:13
                          • Example 5 42:35